Surface Plasmon Resonance is usually regarded as an indiscriminate biosensing technique that monitors the binding of any molecule to the sensor surface, without being able to determine the identity of the binding species. However, using Label-Enhanced SPR, it is possible to discriminate and quantify the binding of two species – one dye-labelled and one unlabelled – simultaneously, even on standard single-wavelength SPR instruments. This feature is very valuable, for example, when studying complex biochemical binding mechanisms, such as competing reactions or allosteric effects. In our new Application Note 024 we present an example on a Biacore™ SPR instrument: the resolution of a mixed, two-species sensorgram into two separate sensorgrams for the two components.
For a copy of the new Application Note 024, or for any other enquiries, please contact email@example.com .