Simple test of EDC/NHS coupling efficiency

Our new Amine Coupling Test Kit offers a simple and accurate way of determining the coupling efficiency of EDC/NHS amine coupling reactions.

Covalent coupling of amine groups to carboxyl groups is swiftly and conveniently mediated through the use of EDC and NHS coupling reagents. The amine coupling is frequently used for conjugation of biomolecules to e.g. gold and latex nanoparticles, biotin, fluorescent dyes, or Episentec™ dyes for Label-Enhanced SPR. More specifically, the amine coupling is often used for immobilizing biomolecules to sensor surfaces, e.g. in SPR methods like Biacore™.

Using our Amine Coupling Test Kit B9005, the coupling efficiency can now be accurately determined with a simple spectrophotometric mix-and-read method.

  • Quality control of EDC/NHS
    • The actual quality of EDC and NHS may vary, leading to varying coupling efficiencies and sometimes insufficient coupling. It is known that the EDC/NHS quality may vary between different vendors and even between different batches.
  • Stability testing of EDC/NHS
    • EDC and NHS are quite unstable. They are prone to hydrolysis by water and it’s difficult to deem the quality of reagents in opened containers. Once dissolved in water, EDC and NHS may be stored for months in the freezer, but it’s recommended to check the quality before using such reagents.
  • Evaluation and optimization of coupling conditions
    • The reaction conditions used for amine coupling may vary. There are many different protocols published, suggesting e.g. different solvents, pH, and reaction times. Using our Amine Coupling Test Kit, different reaction conditions can easily be evaluated and optimized.

For a free download of our Technical Note describing the Amine Coupling Test Kit, please check our Literature page.

For more info or a full price list please contact us at sales@episentum.com.

 

New LE-SPR publication

Label-Enhanced SPR (LE-SPR) can be used to clearly confirm binding site specificity. Brandon Garcia and Brian Geisbrecht at Kansas State University and their co-workers have used LE-SPR in a very elegant manner to study small-molecule inhibitors of the complement system using a standard Biacore™ T200 instrument. Taking advantage of the greatly simplified interpretation of LE-SPR data, binding site specificity was unambiguously confirmed in a way that would have been hard to achieve with conventional SPR.

The full study is recently published in The Journal of Immunology.

For more information about the LE-SPR technology, please contact us directly at info@episentum.com.

 

End-of-year offer: Free Label-Enhanced SPR software

To celebrate the approaching Holiday Season, we’re offering free licenses to our Label-Enhanced SPR software. We want to help more scientists get started with LE-SPR, and we also want to reward our existing customers. So here’s the offer:

  • Buy 1 mg of any Episentec™ dye reagent – get a free 3-month full version license to our EpiGrammer™ software.
  • Buy 2 mg of any Episentec™ dye reagents – get a free 6-month full version license to our EpiGrammer™ software.
  • And, as always, we include generous technical support on the implementation of Label-Enhanced SPR to all our customers.

For any enquiries, please contact us at sales@episentum.com.

 

New Products: Monoclonal Antibodies

Through our collaboration with Biosensor Applications AB, a leader in the development of mobile equipment for drugs and explosives detection, we are now able to offer highly specific, monoclonal antibodies against drugs of abuse. All antibodies are extensively purified using state-of-the art methodology, carefully quality controlled, and functionally characterized using QCM (quartz crystal microbalance) and SAW (surface acoustic wave) biosensors. Recommended applications are biosensors, optical as well as gravimetric, ELISA, and similar applications requiring high quality, primary antibodies.

Please check out our Monoclonal Antibodies page for more info. For any enquiries and orders regarding antibodies, please contact us at: sales@episentum.com.

 

End-of-year offer: Get started with Label-Enhanced SPR

To celebrate the approaching end of year 2015, we are offering the following discounts on any orders on our range of Label-Enhanced SPR products placed before the end of the year:

  • 15% discount off the list price on all Episentec™ dye reagents.
  • 25% discount off the list price on the first annual EpiGrammer™ license.
  • Free support on experimental planning and data evaluation for three months for new customers.

For any additional information, for a quotation, or to place an order, please contact sales@episentum.com.

Download pdf here:   Episentec December 2015

 

New Technical Notes published

We have published four new Technical Notes, i.e. simple guides on the practical implementation of Label-Enhanced SPR. Please check out our Technical Notes page for a complete list of available TechNotes, and please contact sales@episentum.com for a copy of any of the TechNotes.

The new TechNotes are:

Technical Note 007: A Biacore™ MDL method template for inhibition-in-solution (ISA) assays

Technical Note 008: A Biacore™ MDL method template for kinetic assays

These two TechNotes contain ready-to-use methods for customers running MDL methods on e.g. Biacore™ 2000 and Biacore™ 3000. The templates can be used right off, or modified according to the user’s requirements.

Technical Note 009: EpiGrammer™ Tutorial 1: Basic case

Technical Note 010: EpiGrammer™ Tutorial 2: Dual-colour case (two binding species)

These two TechNotes contain simple step-by-step instructions on the use of EpiGrammer™ software. TechNote 009 covers the most basic case, while TechNote 010 covers the case with two simultaneously binding species described in our Application Note 024.

 

Meet Episentec at the Drug Discovery ’15 meeting in Telford, UK, 2-3 September 2015

Take the opportunity to meet with Episentec at the ELRIG Drug Discovery ’15 conference in Telford, UK, 2-3 September 2015. We will present a poster showing how Label-Enhanced SPR improves both the detectability and the specificity in Surface Plasmon Resonance analysis of antibodies and proteins as well as small molecules and fragments.

We will also spend a few days extra in the UK, so if you’re interested in Label-Enhanced SPR and if you’re site is not too far from Telford, please contact us and we’ll arrange a meeting!

Please contact us at sales@episentum.com for any enquiries.