Tag Archives: EpiGrammer

New Technical Notes published

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We have published four new Technical Notes, i.e. simple guides on the practical implementation of Label-Enhanced SPR. Please check out our Technical Notes page for a complete list of available TechNotes, and please contact sales@episentum.com for a copy of any of the TechNotes.

The new TechNotes are:

Technical Note 007: A Biacore™ MDL method template for inhibition-in-solution (ISA) assays

Technical Note 008: A Biacore™ MDL method template for kinetic assays

These two TechNotes contain ready-to-use methods for customers running MDL methods on e.g. Biacore™ 2000 and Biacore™ 3000. The templates can be used right off, or modified according to the user’s requirements.

Technical Note 009: EpiGrammer™ Tutorial 1: Basic case

Technical Note 010: EpiGrammer™ Tutorial 2: Dual-colour case (two binding species)

These two TechNotes contain simple step-by-step instructions on the use of EpiGrammer™ software. TechNote 009 covers the most basic case, while TechNote 010 covers the case with two simultaneously binding species described in our Application Note 024.

 

Dual-colour SPR on a standard Biacore™ instrument

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Surface Plasmon Resonance is usually regarded as an indiscriminate biosensing technique that monitors the binding of any molecule to the sensor surface, without being able to determine the identity of the binding species. However, using Label-Enhanced SPR, it is possible to discriminate and quantify the binding of two species – one dye-labelled and one unlabelled – simultaneously, even on standard single-wavelength SPR instruments. This feature is very valuable, for example, when studying complex biochemical binding mechanisms, such as competing reactions or allosteric effects. In our new Application Note 024 we present an example on a Biacore™ SPR instrument: the resolution of a mixed, two-species sensorgram into two separate sensorgrams for the two components.

For a copy of the new Application Note 024, or for any other enquiries, please contact sales@episentum.com .

 

September offer: Large discounts on Label-Enhanced SPR products

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To help you get a head start in the lab after the summer break, we offer substantial discounts on all orders placed in September 2015:

  • 15% discount on all Episentec™ dye reagents in the B2 and B3 series for Biacore™.
  • 25% discount on our EpiGrammer™ 2.1 software for all new dye customers.

Remember, Label-Enhanced SPR can help you boost the performance of your Surface Plasmon Resonance instrument:

  • 100-fold improved sensitivity – as compared to standard sensorgrams.
  • 100% specificity – with respect to the labelled species.

Please contact sales@episentum.com for any enquiries or to place an order, and claim our September discounts.

Dowload pdf here: Episentec September campaign

 

New EpiGrammer version – Free trial license

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We have developed a new, improved version of the EpiGrammer™ software for Label-Enhanced SPR, and celebrate this with a special offer:

A free 3-month EpiGrammer trial license to all new Episentec™ dye reagent customers in April 2015.

EpiGrammer software is used for calculating enhanced sensorgrams based on our proprietary Label-Enhanced SPR technology:

  • 100-fold improved sensitivity as compared to standard SPR sensorgrams.
  • 100% specificity with respect to the dye-labelled species.

News in version 2.1:

  • Extended linear dynamic range and more efficient rejection of large disturbances.
  • Improved quantification of small binding levels in presence of varying DMSO concentrations.
  • The interactive, easy-to-use graphical interface and the data import/export routines are unchanged.

Please contact sales@episentum.com for any enquiries or to place an order.