Our new Amine Coupling Test Kit offers a simple and accurate way of determining the coupling efficiency of EDC/NHS amine coupling reactions.
Covalent coupling of amine groups to carboxyl groups is swiftly and conveniently mediated through the use of EDC and NHS coupling reagents. The amine coupling is frequently used for conjugation of biomolecules to e.g. gold and latex nanoparticles, biotin, fluorescent dyes, or Episentec™ dyes for Label-Enhanced SPR. More specifically, the amine coupling is often used for immobilizing biomolecules to sensor surfaces, e.g. in SPR methods like Biacore™.
Using our Amine Coupling Test Kit B9005, the coupling efficiency can now be accurately determined with a simple spectrophotometric mix-and-read method.
- Quality control of EDC/NHS
- The actual quality of EDC and NHS may vary, leading to varying coupling efficiencies and sometimes insufficient coupling. It is known that the EDC/NHS quality may vary between different vendors and even between different batches.
- Stability testing of EDC/NHS
- EDC and NHS are quite unstable. They are prone to hydrolysis by water and it’s difficult to deem the quality of reagents in opened containers. Once dissolved in water, EDC and NHS may be stored for months in the freezer, but it’s recommended to check the quality before using such reagents.
- Evaluation and optimization of coupling conditions
- The reaction conditions used for amine coupling may vary. There are many different protocols published, suggesting e.g. different solvents, pH, and reaction times. Using our Amine Coupling Test Kit, different reaction conditions can easily be evaluated and optimized.
For a free download of our Technical Note describing the Amine Coupling Test Kit, please check our Literature page.
For more info or a full price list please contact us at email@example.com.
The Biophysics group at Genentech has published a new paper on the use of ‘chaser’ assays to study extremely long dissociation times, which has been added to our Scientific Publications page. The corresponding poster was honoured with the poster award at the recent GE Healthcare DiPIA 2018 conference in Boston.
The Episentum office is open for customer service all summer except weeks 30-31 (23 July – 3 August).
We would like to wish all our Customers, Clients, Companions, and Contacts a pleasant, dye-enhanced summer with a yellow sun, blue skies, green trees, and the odd white cloud.
We have launched a new version of the EpiGrammer™ software that improves the performance even of top notch SPR instruments. Contact us on firstname.lastname@example.org for a copy of our latest Application Note 26 to learn how the new version improves the signal-to-noise ratio and reduces the disturbance level of the Biacore™ T200 SPR instrument.
The new EpiGrammer 3.0 version uses improved algorithms for enhanced performance of Biacore™ T100/T200 systems. We are offering a limited number of free trial licenses to the pilot version of EpiGrammer 3.0 to selected customers. If you’re interested, please contact us on email@example.com.
Please check out our new web page listing scientific publications relating to Label-Enhanced SPR and some SPR publications of general background interest!
For any new Episentec™ dye reagent orders placed before the end of 2017, we are happy to offer a 20% discount plus a free one-month trial license to the EpiGrammer™ LE-SPR software.
To place an order or for any other enquiries, please contact us at firstname.lastname@example.org.
We are now offering a Label-Enhanced SPR Validation Kit B9003 to be used as a learning tool or test tool for Biacore™ users that want to get a first acquaintance to the LE-SPR technology. The Validation Kit can also be used to test a SPR instrument, or as a positive control sample during regular LE-SPR analysis. More information on the Validation Kit is found in our recently published Episentum Technical Note 012.
To get a free copy of our Technical Note 012 or for any other enquiries, please contact us at email@example.com.
Episentum will present a poster on Label-Enhanced SPR at the NovAlix conference Biophysics in Drug Discovery, Strasbourg, France, 6-9 June 2017. We will display the enhanced sensitivity in small molecule analysis, as well as the enhanced specificity and the dual-colour capabilities in protein analysis.
We’re looking forward to meeting with lots of SPR scientists and other biophysicists, including old and new customers. For any information on LE-SPR prior to the meeting, or to book a one-to-one meeting, please contact us at firstname.lastname@example.org.
Label-Enhanced SPR (LE-SPR) can be used to clearly confirm binding site specificity. Brandon Garcia and Brian Geisbrecht at Kansas State University and their co-workers have used LE-SPR in a very elegant manner to study small-molecule inhibitors of the complement system using a standard Biacore™ T200 instrument. Taking advantage of the greatly simplified interpretation of LE-SPR data, binding site specificity was unambiguously confirmed in a way that would have been hard to achieve with conventional SPR.
The full study is recently published in The Journal of Immunology.
For more information about the LE-SPR technology, please contact us directly at email@example.com.
Episentum will present a poster at the Advances in Drug Discovery meeting, Cambridge, UK, 6-7 March 2017. The poster will demonstrate how Label-Enhanced SPR can improve the surface plasmon resonance analysis of small molecules as well as larger biomolecules.
We’re looking forward to meeting with lots of SPR users, including old and new customers. For any information on LE-SPR prior to the meeting, or to book a one-to-one meeting, please contact us at firstname.lastname@example.org.